Research Assistant (Immunobiology/Islet Cell & Regenerative Biology) (Finance)

Overview

The Research Assistant assists with experiments in a basic research lab with a focus on genetic diseases and autoimmunity. Duties in this new lab will include performing molecular and cell biology experiments independently and as part of a team, as well as general lab maintenance and ordering supplies, and genotyping and maintaining a mouse colony. Experiments will include working with live mice, mouse and human cells in culture, as well as basic molecular biology, cell biology and biochemistry.

As a health care organization, we have the responsibility to do everything in our power to care for and protect our patients, our colleagues and our communities. We require that all staff be vaccinated against influenza (flu) and COVID-19 as a condition of employment.

Responsibilities

• The successful applicant must be able to design and execute experiments with appropriate controls and experimental rigor and to interpret the derived data.
• Genotype transgenic and knock-out mice using PCR and flow cytometry.
• Perform fluorescence activated cell sorting to analyze and purify live cells.
• Maintain sterile cultures of rodent and human primary cells and cell lines.
• Maintain a specific pathogen free mouse colony, including breeding, weighing, and monitoring food intake and response to medication.
• Perform in vivo experiments and harvesting of cells and tissues from mice.
• Provide general lab support (ordering and stocking supplies, coordinating glassware washing, equipment repairs, buffer preparation, etc.)
• Maintain accurate and up-to-date records and data storage/archiving.
• Assist PI and other lab members in organizing, maintaining and distributing plasmids, antibodies, cell lines, etc.
• Ensure safe laboratory practices and compliance with all institutional research guidelines.
• Other duties as assigned.

Qualifications

• B.S. in Biochemistry, Biology, Molecular Biology or related fields
• Strong background in immunology, molecular and cell biology and/or biochemistry.
• Experience working with animals is preferred
• Excellent organizational skills, interpersonal skills, and a high level of motivation and reliability